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- Speed. Automate your entire analysis and get results in less than a minute per image.
- Objectivity. Unmatched automation reduces the need for manual intervention and minimises inter-operator variability.
- Reproducibility. Consistent analysis results between different users, labs and different imaging devices.
- Quantitation. Enables reliable, accurate quantitation of gel and blot images.
- 21 CFR/GMP-compliant Meet FDA 21 CFR part 11/EU Annex 11/GAMP5 GMP compliance for regulated pharmaceutical environments, from captured image through to final analysis and report production.
Automatic detection and quantification of gel electrophoresis data
Phoretix 1D offers automatic lane and band detection or, for even deeper automation and standardisation of your workflow, user-defined protocols which allow full one-click analysis of gel or Western blot images. This removes the potential for operator error entirely.
Coupled with intuitive background removal using industry-leading algorithms, molecular weight calculation, quantity calibration and normalisation Phoretix 1D has all the modern tools required for comprehensive experimental analysis.
Advanced background removal and noise correction
Background
noise can come from various sources, such as non-specific binding of
antibodies or stains, uneven illumination, dust, scratches, or
instrument artifacts. If not corrected, background noise can affect the
intensity and shape of the bands or spots, leading to inaccurate
measurements of their size, quantity, and relative expression.
Therefore, it is important to perform background removal to subtract
the unwanted signal from the image, before quantifying bands or spots.
Our Phoretix 1D software features multiple methods of background
removal for our users to choose from and refine their analysis:
Adaptive
- Rolling Ball
- Lane Edge
- Rubber Band
- None
- Constant
- Image Rectangle
- Profile Minimum
Analyse a huge variety of experiments
Phoretix 1D is the perfect choice for the quantitative analysis of:
- DNA/RNA Gels
- Western Blots
- Northern Blots
- Southern Blots
- Thin-layer chromatography
- Lateral flow tests (LFT/LFA)
Harmonise your lab equipment
Our software is device agnostic, so can reduce costs as it can be used with all of your existing laboratory equipment or any additional equipment gained during business acquisitions, it also works with all industry-standard image formats:.TIF/.TIFF
Phoretix 1D supports 8-bit, 16-bit (recommended) and higher uncompressed TIFF images. Multi-page and multiplex TIFFs are supported and each page is interpreted as a “channel” (all of which must have the same dimensions and pixel format).
.JPG/.JPEG/.PNG/.BMP/.IMG
While not recommended because of their low bit-depth and (sometimes) compression artifacts, these image formats can still be analysed.
This means you aren’t locked in or reliant on one particular equipment manufacturer, reducing your business risk, allowing you greater freedom in your imaging equipment selection and facilitating easier tech transfer between labs.