TotalLab is a specialist software developer for biotech, pharmaceuticals, and life science industries, boasting over 20 years of experience. TotalLab offers advanced software services for equipment manufacturers, bespoke solutions for life science companies, and off-the-shelf products to tackle scientific challenges, automate workflows, and enable FDA 21 CFR Part 11/GMP regulatory compliance. Totallab provides the following software:

Phoretix 1D Phoretix Array Phoretix Colony Phoretix Toolbox CLIQS 1D Pro SameSpots SpotMap

Automatic Gel Electrophoresis and Western Blot Analysis Software

Phoretix 1D is the latest evolution of our 1D software and has been developed in line with industry needs and feedback to deliver the highest-quality data in the shortest amount of time. We have distilled our twenty years’ worth of experience supporting the life science industry into Phoretix 1D to deliver our most advanced set of analysis tools yet to analyse images derived from any Gel Documentation, densitometer or other imaging devices.
Phoretix 1D is the complete solution for analysing gel electrophoresis and immunoblot images, it offers:
    • Speed. Automate your entire analysis and get results in less than a minute per image.
    • Objectivity. Unmatched automation reduces the need for manual intervention and minimises inter-operator variability.
    • Reproducibility. Consistent analysis results between different users, labs and different imaging devices.
    • Quantitation. Enables reliable, accurate quantitation of gel and blot images.
    • 21 CFR/GMP-compliant Meet FDA 21 CFR part 11/EU Annex 11/GAMP5 GMP compliance for regulated pharmaceutical environments, from captured image through to final analysis and report production.  
    Phoretix 1D is compatible with the latest versions of both Windows (currently Windows 11) and macOS operating systems (up to and including Ventura and M1/M2/M3 processors).

Automatic detection and quantification of gel electrophoresis data

Phoretix 1D offers automatic lane and band detection or, for even deeper automation and standardisation of your workflow, user-defined protocols which allow full one-click analysis of gel or Western blot images. This removes the potential for operator error entirely.

Coupled with intuitive background removal using industry-leading algorithms, molecular weight calculation, quantity calibration and normalisation Phoretix 1D has all the modern tools required for comprehensive experimental analysis.

Advanced background removal and noise correction

Background noise can come from various sources, such as non-specific binding of antibodies or stains, uneven illumination, dust, scratches, or instrument artifacts. If not corrected, background noise can affect the intensity and shape of the bands or spots, leading to inaccurate measurements of their size, quantity, and relative expression. Therefore, it is important to perform background removal to subtract the unwanted signal from the image, before quantifying bands or spots.
Our Phoretix 1D software features multiple methods of background removal for our users to choose from and refine their analysis:


  • Rolling Ball
  • Lane Edge
  • Rubber Band
  • None
  • Constant
  • Image Rectangle
  • Profile Minimum

It also features innovative noise-reduction techniques to smooth out the intensity signal of your bands or spots, resulting in cleaner data for analysis.

Analyse a huge variety of experiments

Phoretix 1D is the perfect choice for the quantitative analysis of:

  • DNA/RNA Gels
  • Western Blots
  • Northern Blots
  • Southern Blots
  • Thin-layer chromatography
  • Lateral flow tests (LFT/LFA)

Harmonise your lab equipment

Our software is device agnostic, so can reduce costs as it can be used with all of your existing laboratory equipment or any additional equipment gained during business acquisitions, it also works with all industry-standard image formats:
Phoretix 1D supports 8-bit, 16-bit (recommended) and higher uncompressed TIFF images. Multi-page and multiplex TIFFs are supported and each page is interpreted as a “channel” (all of which must have the same dimensions and pixel format).
While not recommended because of their low bit-depth and (sometimes) compression artifacts, these image formats can still be analysed.
This means you aren’t locked in or reliant on one particular equipment manufacturer, reducing your business risk, allowing you greater freedom in your imaging equipment selection and facilitating easier tech transfer between labs.

Supporting 21 CFR Part 11 compliance in GxP laboratories/environments

Phoretix 1D, along with all TotalLab software, can be integrated into our 21 CFR Part 11/GMP-compliant AuditSafe software to generate a full audit trail with electronic signatures during analysis for use in regulated industry environments where data traceability, accountability, and integrity are critical.

Click Here to know more about the software Phoretix Array                                                     Contact Us for purchasing a license for the Phoretix Array